Journal: iScience

Article Title: Aquaporin-8 ameliorates hepatic steatosis through farnesoid X receptor in obese mice

doi: 10.1016/j.isci.2023.106561

Figure Lengend Snippet: Downregulation of AQP8 in the livers of NAFLD patients and obese mice and effects of hepatic AQP8 overexpression in vitro Hepatic mRNA and protein levels of AQP8 decreased in patients and obese mouse model: (A) NAFLD patients (n = 8 per group), (B) db/db mice (n = 6 per group), (C) high fat diet (HFD) mice (n = 7 per group), (D) short-term HFD mice (n = 5 per group) (See also Figure S2 A), (E) HepG2 cells without or with FFA(OA:PA = 2:1) treatment for the indicated time(See also Figure S2 B). Primary mouse hepatocytes (PMHs) and HepG2 cells were transfected with either ad-GFP or ad-AQP8 with or without FFA treatment. (F) AQP8 mRNA expression of PMHs. (G) Analysis of AQP8 and lipogenic genes expression (See also Figure S2 C). (H) Oil red O staining (200 X) of HepG2 cells. (I) TG measurement of PMHs. The data represent the mean ± SEM values. ∗p < 0.05 ∗∗p < 0.01 ∗∗∗p < 0.001.

Article Snippet: Mouse primary hepatocytes and HepG2 cells (AmericanType Culture Collection, China) were cultured in DMEM supplemented with 10% of fetal bovine serum, 1% of L-glutamine, and 1% of penicillin-streptomycin solution and maintained in a humidified atmosphere of 5% CO2 at 37°C.

Techniques: Over Expression, In Vitro, Transfection, Expressing, Staining

Journal: iScience

Article Title: Aquaporin-8 ameliorates hepatic steatosis through farnesoid X receptor in obese mice

doi: 10.1016/j.isci.2023.106561

Figure Lengend Snippet: FXR regulates AQP8 expression and FXR knockout inhibits the improvement of AQP8 in NAFLD (A) PMHs were treated with DMSO, PPARα agonist Fenofibrate, PPARβ/δ agonist GW0742, PPARγ agonist Rosiglitazone, FXR agonist GW4064, LXR agonist T0901317 and RT–PCR analysis of AQP8 gene expression. (B) Dual-luciferase reporter assay was conducted to determine the effect of FXR agonist GW4064 on AQP8 activity. (C) Dual-luciferase reporter assay was conducted to determine the effect of FXR overexpression on AQP8 activity. (D) The interaction of FXR with AQP8 promoter containing putative FXRE was confirmed by chromatin immunoprecipitation (ChIP) in HepG2 cells (n = 3 individual experiments) using an FXR antibody, IgG was used as a negative control. (E) Relative AQP8 mRNA levels in the livers of FXR wild-type (WT) and knockout (KO) mice. (F) FXR-WT and FXR-KO were fed an HFD for two weeks and then i.v injected with either ad-GFP or ad-AQP8 and relative AQP8 mRNA levels in the livers of three groups. (G–J) Serum levels of TBA, TG, TC, LDL-c. (K) The analysis of liver weight in three groups. The count is liver weight per kilogram of body weight. (L) Quantification of hepatic TG contents. (M) Histological analysis of hematoxylin-eosin-stained liver sections, magnification: 200×, scale bar, 100 μm. The data represent the mean ± SEM values. ∗p<0.05 ∗∗p<0.01 ∗∗∗p<0.001.

Article Snippet: Mouse primary hepatocytes and HepG2 cells (AmericanType Culture Collection, China) were cultured in DMEM supplemented with 10% of fetal bovine serum, 1% of L-glutamine, and 1% of penicillin-streptomycin solution and maintained in a humidified atmosphere of 5% CO2 at 37°C.

Techniques: Expressing, Knock-Out, Reverse Transcription Polymerase Chain Reaction, Gene Expression, Luciferase, Reporter Assay, Activity Assay, Over Expression, Chromatin Immunoprecipitation, Negative Control, Injection, Staining

Journal: iScience

Article Title: Aquaporin-8 ameliorates hepatic steatosis through farnesoid X receptor in obese mice

doi: 10.1016/j.isci.2023.106561

Figure Lengend Snippet:

Article Snippet: Mouse primary hepatocytes and HepG2 cells (AmericanType Culture Collection, China) were cultured in DMEM supplemented with 10% of fetal bovine serum, 1% of L-glutamine, and 1% of penicillin-streptomycin solution and maintained in a humidified atmosphere of 5% CO2 at 37°C.

Techniques: Virus, Software

Journal: The Journal of Pharmacology and Experimental Therapeutics

Article Title: Metabolism of Beclomethasone Dipropionate by Cytochrome P450 3A Enzymes

doi: 10.1124/jpet.112.202556

Figure Lengend Snippet: (A) Quantification of BDP metabolites produced by A549 cells treated with BDP or BDP with esterase inhibitors (+EI). (B) Relative quantification of BDP metabolites produced by DPX2 cells treated with BDP or BDP with esterase inhibitors (+EI). Data are the mean and standard deviation from six replicates. n.d. Signifies that the metabolite was not detected. (C and D) CYP3A enzyme mRNA abundance, measured by qPCR in A549 (C) and DPX2 (D) cells. Data are represented as the number of mRNA copies per 10,000 copies of β2-macroglobulin (a “housekeeping” gene). Statistics used for A549 cell data analysis were one-way analysis of variance with Dunnett’s post-hoc test. For DPX2 cell data analysis two-way ANOVA with Bonferronni post-hoc testing was used. Data are the mean and standard deviation from 6 replicates. n.d. Signifies that mRNA was not detected. *P < 0.05; ***P < 0.001; ****P < 0.0001.

Article Snippet: DPX2 cells (HepG2 background with human PXR stably overexpressed to drive the expression of a CYP3A4 reporter gene construct by PXR agonists) were provided by Dr. Judy Raucy (Puracyp Inc., Carlsbad, CA).

Techniques: Produced, Quantitative Proteomics, Standard Deviation

Journal: Antioxidants

Article Title: Discovery of Eicosapentaenoic Acid Esters of Hydroxy Fatty Acids as Potent Nrf2 Activators

doi: 10.3390/antiox9050397

Figure Lengend Snippet: Evaluation of the antioxidant potential of eicosapentaenoic (EPA)-derived FAHFAs in C3A cells. ( A ) Cell viabilities (IC 50 ) of 12-EPAHSA and 12-EPAHOA ( B ) Reporter gene assay results of 12-EPAHSA and 12-EPAHOA ( C ) Relative nuclear Nrf2 protein expression induced by 12-EPAHSA ( D ) Protein levels of Nrf2 by Western blot analysis after treated with 12-EPAHSA. ( E) Relative expression of antioxidant enzymes in response to 12-EPAHSA treatment. * p < 0.05, ** p < 0.01, *** p < 0.001, ns: not significant (one-way ANOVA) ( n = 6). (NQO1: NAD(P)H quinone oxidoreductase 1, HO1: heme oxygenase-1, GCLM: glutamate–cysteine ligase modulatory subunit, GCLC: glutamate–cysteine ligase catalytic subunit, CAT: catalase, and SOD1: superoxide dismutase 1).

Article Snippet: The C3A cells (derivative of Human HepG2, CRL-10741) were purchased from ATCC (Manassas, VA, USA) and kept at 37 °C in an incubator under a humidified atmosphere of 5% CO 2 using minimum essential medium (MEM) with 10% ( v/v ) fetal bovine serum (FBS), and 1% Penicillin–Streptomycin–Neomycin mixture (all were obtained from Thermo Fisher Scientific, Japan).

Techniques: Derivative Assay, Reporter Gene Assay, Expressing, Western Blot

Journal: Antioxidants

Article Title: Discovery of Eicosapentaenoic Acid Esters of Hydroxy Fatty Acids as Potent Nrf2 Activators

doi: 10.3390/antiox9050397

Figure Lengend Snippet: Effect of 12-EPAHSA on oxidation of LA-induced LDs in C3A cells. Fluorescent images showed neutral lipid (red), lipid peroxide (green) and nuclei (blue) ( A ). The scale bar shown in each image is 10 µm. To quantify the number of total LDs and oxLDs, fluorescent images were analyzed with ImageJ software. The results are presented as the number of total LDs ( B1 ), the number of oxLDs ( B2 ), and degree of oxidation ( B3 ) for small (< 3 µm 2 ) and large (≥ 3 µm 2 ) LDs. Columns and bars represent the mean ± SEM ( n = 3). ** p < 0.01, *** p < 0.001.

Article Snippet: The C3A cells (derivative of Human HepG2, CRL-10741) were purchased from ATCC (Manassas, VA, USA) and kept at 37 °C in an incubator under a humidified atmosphere of 5% CO 2 using minimum essential medium (MEM) with 10% ( v/v ) fetal bovine serum (FBS), and 1% Penicillin–Streptomycin–Neomycin mixture (all were obtained from Thermo Fisher Scientific, Japan).

Techniques: Software

Journal: Antioxidants

Article Title: Discovery of Eicosapentaenoic Acid Esters of Hydroxy Fatty Acids as Potent Nrf2 Activators

doi: 10.3390/antiox9050397

Figure Lengend Snippet: Biosynthesis of 12-EPAHSA in C3A cells spiked with concentrations of single components of the equimolar mixture of EPA and 12-HSA.

Article Snippet: The C3A cells (derivative of Human HepG2, CRL-10741) were purchased from ATCC (Manassas, VA, USA) and kept at 37 °C in an incubator under a humidified atmosphere of 5% CO 2 using minimum essential medium (MEM) with 10% ( v/v ) fetal bovine serum (FBS), and 1% Penicillin–Streptomycin–Neomycin mixture (all were obtained from Thermo Fisher Scientific, Japan).

Techniques:

Journal: Bioscience Reports

Article Title: Promoter analysis of the DHCR24 (3β-hydroxysterol Δ 24 -reductase) gene: characterization of SREBP (sterol-regulatoryelement-binding protein)-mediated activation

doi: 10.1042/BSR20120095

Figure Lengend Snippet: HepG2 cells were incubated with lovastatin (Lov, 10 μM) or LDL (30 μg of cholesterol/ml) in a lipoprotein deficient medium. Samples were harvested after 24 h and the expression of DHCR24 ( A ) and HMGCR ( B ) was measured by real-time RT–PCR using the relative quantification method by comparison with the expression of the housekeeping gene RPLP0 . The results are expressed as the relative amount of mRNA compared with the level under control conditions. Results are the means±S.E.M. for three independent experiments. * P <0.05 and ** P <0.01 by the paired Student's t test.

Article Snippet: The promoter region of the human DHCR24 gene was generated by PCR amplification using HepG2 cell genomic DNA as a template and Platinum® Pfx DNA Polymerase (Invitrogen).

Techniques: Incubation, Expressing, Quantitative RT-PCR, Quantitative Proteomics, Comparison, Control

Journal: Bioscience Reports

Article Title: Promoter analysis of the DHCR24 (3β-hydroxysterol Δ 24 -reductase) gene: characterization of SREBP (sterol-regulatoryelement-binding protein)-mediated activation

doi: 10.1042/BSR20120095

Figure Lengend Snippet: A fragment of 1018 bp of the 5′ flanking region containing the promoter of DHCR24 was cloned into the pGL3-basic vector as described in the Experimental section. Unidirectional serial deletions of the pH DHCR24 construct were generated and various reporter gene plasmids were transfected into HepG2 ( A ) or SK-N-MC ( B ) cells. Plasmid constructs containing different sequential portions of the DHCR24 promoter are schematized on the left and their corresponding luciferase activities are shown in the histograms on the right. The transcription factor-binding sites are identified. Cholesterol availability was modified by the incubation of cells with lovastatin (Lov, 10 μM) or LDL (30 μg of cholesterol/ml) for 24 h. Transcriptional activation was measured by the dual-luciferase reporter assay. Bars indicate the relative firefly luciferase activity normalized to Renilla luciferase activity used as the internal control of transfection efficiency. Results are the means±S.E.M. for three independent experiments.

Article Snippet: The promoter region of the human DHCR24 gene was generated by PCR amplification using HepG2 cell genomic DNA as a template and Platinum® Pfx DNA Polymerase (Invitrogen).

Techniques: Clone Assay, Plasmid Preparation, Construct, Generated, Transfection, Luciferase, Binding Assay, Modification, Incubation, Activation Assay, Reporter Assay, Activity Assay, Control

Journal: Bioscience Reports

Article Title: Promoter analysis of the DHCR24 (3β-hydroxysterol Δ 24 -reductase) gene: characterization of SREBP (sterol-regulatoryelement-binding protein)-mediated activation

doi: 10.1042/BSR20120095

Figure Lengend Snippet: ( A ) Sequence of the consensus SRE, SRE in the DHCR24 promoter and in the mutated DHCR24 promoter. ( B ) Transcriptional activity assay of the wild-type and mutated SRE in the DHCR24 promoter. The wildtype or the SRE-mutated vectors were transfected into HepG2 cells. Cholesterol availability was modified by the incubation of cells with lovastatin (Lov, 10 μM) or LDL (30 μg of cholesterol/ml) for 24 h, and transcriptional activation was measured by the dual-luciferase reporter assay. Bars indicate the relative firefly luciferase activity normalized to Renilla luciferase activity used as an internal control of transfection efficiency. Results are the means±S.E.M. for three independent experiments. *** P <0.001 against different treatment within the same plasmid; # P <0.05 and ### P <0.001 against pH DHCR24 within the same treatment by two-way ANOVA.

Article Snippet: The promoter region of the human DHCR24 gene was generated by PCR amplification using HepG2 cell genomic DNA as a template and Platinum® Pfx DNA Polymerase (Invitrogen).

Techniques: Sequencing, Activity Assay, Transfection, Modification, Incubation, Activation Assay, Luciferase, Reporter Assay, Control, Plasmid Preparation

Journal: Bioscience Reports

Article Title: Promoter analysis of the DHCR24 (3β-hydroxysterol Δ 24 -reductase) gene: characterization of SREBP (sterol-regulatoryelement-binding protein)-mediated activation

doi: 10.1042/BSR20120095

Figure Lengend Snippet: HepG2 cells incubated with lovastatin (Lov, 10 μM) or LDL (30 μg of cholesterol/ml) were subjected to ChIP using an antibody against SREBP-2 or IgG as a negative control. Co-precipitated DNA was amplified by PCR using primers flanking the SRE-binding site in the DHCR24 promoter as described in the Experimental section. The specific amplification of the immunoprecipitated SRE of the LDLR promoter was used as a positive control. Results are the means±S.E.M. for four independent experiments. * P <0.05 against the control condition by the paired Student's t test.

Article Snippet: The promoter region of the human DHCR24 gene was generated by PCR amplification using HepG2 cell genomic DNA as a template and Platinum® Pfx DNA Polymerase (Invitrogen).

Techniques: Incubation, Negative Control, Amplification, Binding Assay, Immunoprecipitation, Positive Control, Control

Journal: European journal of biochemistry

Article Title: Functional analysis of the rat bile salt export pump gene promoter.

doi: 10.1046/j.1432-1033.2002.03030.x

Figure Lengend Snippet: Fig. 3. Deletional analysis of the rat Bsep promoter activity in trans- fected HepG2 cells. Varying lengths of the 5¢ region of the Bsep gene, from )1453 to )27 bp relative to the transcription start site and extending to nucleotide +80, were amplified by PCR using the genomic clone as a template and then inserted into the promoterless luciferase vector pGL3 basic. The plasmid p+80–1453 containing the same nucleotide sequence in antisense orientation served as a control. The constructs were transiently cotransfected with a renilla luciferase expression plasmid (pRL-TK) into HepG2 cells as described in Materials and methods. Luciferase activity of each construct was determined as relative light units of firefly luciferase per relative light units of renilla luciferase (luc/ren). All values were expressed relative to the longest construct p-1453, which was assigned 100%. Transfections were carried out in triplicate, and repeated three times. Data are the means ± 1 SD.

Article Snippet: Cell culture, transient transfections and reporter gene assays Human hepatoblastoma HepG2 (HB-8065, ATCC), colon carcinoma CaCo2 (ACC169, DSMZ), Madin–Darby canine kidney (MDCK; Dr Birchmeier, Max Delbrück Center of Molecular Medicine, Berlin, Germany) and mouse fibroblast NIH 3T3 cells (Dr Blankenstein, Max Delbrück Center of Molecular Medicine, Berlin, Germany) were cultured in Dulbecco’s modified Eagle medium containing 10% fetal bovine serum, 1% nonessential amino acids, 1 mmolÆL)1 sodium pyruvate, and 2 mmolÆL)1 glutamine.

Techniques: Activity Assay, Luciferase, Plasmid Preparation, Sequencing, Control, Construct, Expressing, Transfection

Journal: European journal of biochemistry

Article Title: Functional analysis of the rat bile salt export pump gene promoter.

doi: 10.1046/j.1432-1033.2002.03030.x

Figure Lengend Snippet: Fig. 5. Effect of bile acids on Bsep promotor function in HepG2 cells. (A) Reporter gene activity after transfection in triplicate with p-1453, the longest construct containing all upstream regulatory elements. Cells were subsequently incubated for 48 h with the indicated bile acids at the concentrations stated in Materials and methods. Promotor activities were determined relative to untreated controls which were set 100%. Data are the means ± SD of at least three individual experi- ments. (B) Transfection with p-126, the minimal Bsep promoter con- taining one FXRE close to the TATA motif. Incubation with bile acids and assays of promotor activities were carried out as in (A). *P < 0.05.

Article Snippet: Cell culture, transient transfections and reporter gene assays Human hepatoblastoma HepG2 (HB-8065, ATCC), colon carcinoma CaCo2 (ACC169, DSMZ), Madin–Darby canine kidney (MDCK; Dr Birchmeier, Max Delbrück Center of Molecular Medicine, Berlin, Germany) and mouse fibroblast NIH 3T3 cells (Dr Blankenstein, Max Delbrück Center of Molecular Medicine, Berlin, Germany) were cultured in Dulbecco’s modified Eagle medium containing 10% fetal bovine serum, 1% nonessential amino acids, 1 mmolÆL)1 sodium pyruvate, and 2 mmolÆL)1 glutamine.

Techniques: Activity Assay, Transfection, Construct, Incubation

Journal: European journal of biochemistry

Article Title: Functional analysis of the rat bile salt export pump gene promoter.

doi: 10.1046/j.1432-1033.2002.03030.x

Figure Lengend Snippet: Fig. 6. Decrease of basal activity and loss of CDCA-mediated stimu- lation of the minimal Bsep promoter after mutation of the FXRE. The wild-type (p-126) or the mutated (m-126) minimal Bsep reporter plasmid were transfected into HepG2 cells, as described in Fig. 5. Luciferase activities were determined following a 48 h incubation in the presence or absence of 100 lM CDCA. *P < 0.05.

Article Snippet: Cell culture, transient transfections and reporter gene assays Human hepatoblastoma HepG2 (HB-8065, ATCC), colon carcinoma CaCo2 (ACC169, DSMZ), Madin–Darby canine kidney (MDCK; Dr Birchmeier, Max Delbrück Center of Molecular Medicine, Berlin, Germany) and mouse fibroblast NIH 3T3 cells (Dr Blankenstein, Max Delbrück Center of Molecular Medicine, Berlin, Germany) were cultured in Dulbecco’s modified Eagle medium containing 10% fetal bovine serum, 1% nonessential amino acids, 1 mmolÆL)1 sodium pyruvate, and 2 mmolÆL)1 glutamine.

Techniques: Activity Assay, Mutagenesis, Plasmid Preparation, Transfection, Luciferase, Incubation

Journal: European journal of biochemistry

Article Title: Functional analysis of the rat bile salt export pump gene promoter.

doi: 10.1046/j.1432-1033.2002.03030.x

Figure Lengend Snippet: Fig. 8. Effects of endogenous substrates and drugs on Bsep promoter function. The full length Bsep reporter plasmid (p-1453) was trans- fected into HepG2 cells and luciferase activities were measured after a 48 h incubation period in the presence of the indicated compounds. The following substrate concentrations were used: 5 lmolÆL)1 each of dexamethasone, hydrocortisone, and b-estradiol; 50 lmolÆL)1 each of phenobarbital, rifampin, and tamoxifen. Assay conditions were the same as described in Fig. 5. *P < 0.05.

Article Snippet: Cell culture, transient transfections and reporter gene assays Human hepatoblastoma HepG2 (HB-8065, ATCC), colon carcinoma CaCo2 (ACC169, DSMZ), Madin–Darby canine kidney (MDCK; Dr Birchmeier, Max Delbrück Center of Molecular Medicine, Berlin, Germany) and mouse fibroblast NIH 3T3 cells (Dr Blankenstein, Max Delbrück Center of Molecular Medicine, Berlin, Germany) were cultured in Dulbecco’s modified Eagle medium containing 10% fetal bovine serum, 1% nonessential amino acids, 1 mmolÆL)1 sodium pyruvate, and 2 mmolÆL)1 glutamine.

Techniques: Plasmid Preparation, Luciferase, Incubation

Journal: Cancer Cell International

Article Title: ARNT is a potential direct HIF-1 target gene in human Hep3B hepatocellular carcinoma cells

doi: 10.1186/s12935-017-0446-2

Figure Lengend Snippet: CRISPR/Cas9 genome editing of the ARNT gene promoter. a Location of CRISPR/Cas9 target sequences ( light grey ) within Region 3. HBS HIF binding site, HAS HIF ancillary sequence, PAM protospacer-adjacent motif. b Cleavage detection assay of untransfected (UT), CRISPR/Cas9-Target 1 (T1) or CRISPR/Cas9-Target 2 (T2) transfected Hep3B cells. Fragment sizes are given in bp. The arrow indicates the re-hybridised PCR product. Representative result of n = 3 independent experiments. c. p. cleavage products

Article Snippet: Human hepatocellular carcinoma Hep3B cells (ATCC) were maintained in RPMI 1640 medium (Gibco) containing 10% FBS and Penicillin/Streptomycin.

Techniques: CRISPR, Binding Assay, Sequencing, Detection Assay, Transfection

Journal: Cancer Cell International

Article Title: ARNT is a potential direct HIF-1 target gene in human Hep3B hepatocellular carcinoma cells

doi: 10.1186/s12935-017-0446-2

Figure Lengend Snippet: Western blot analysis of CRISPR/Cas9 genome edited Hep3B cells. a Representative Western blot of n = 4 independent experiments. Untransfected (UT), CRISPR/Cas9-Target 1 (T1) or CRISPR/Cas9-Target 2 (T2) transfected Hep3B cells were cultured under normoxic (N) conditions or exposed to hypoxia (H) for 8 h. Protein masses are indicated in kDa. b Densitometry of ARNT protein level (corresponding to a ) normalised to Lamin A/C. Values are presented as mean ± SEM of n = 4 independent experiments

Article Snippet: Human hepatocellular carcinoma Hep3B cells (ATCC) were maintained in RPMI 1640 medium (Gibco) containing 10% FBS and Penicillin/Streptomycin.

Techniques: Western Blot, CRISPR, Transfection, Cell Culture

Journal: Cancer Cell International

Article Title: ARNT is a potential direct HIF-1 target gene in human Hep3B hepatocellular carcinoma cells

doi: 10.1186/s12935-017-0446-2

Figure Lengend Snippet: qRT-PCR analysis of CRISPR/Cas9 genome-edited Hep3B cells cultured in Lumox ® gas permeable petri-dishes. a ARNT mRNA- and b HIF1A mRNA expression were measured using TaqMan ® Gene Expression Assays under normoxic and hypoxic (3% O 2 , 5 h) conditions. Values are presented as mean ± SEM of n = 3 independent experiments. N normoxia, H hypoxia

Article Snippet: Human hepatocellular carcinoma Hep3B cells (ATCC) were maintained in RPMI 1640 medium (Gibco) containing 10% FBS and Penicillin/Streptomycin.

Techniques: Quantitative RT-PCR, CRISPR, Cell Culture, Expressing, Gene Expression

Journal: Cancer Cell International

Article Title: ARNT is a potential direct HIF-1 target gene in human Hep3B hepatocellular carcinoma cells

doi: 10.1186/s12935-017-0446-2

Figure Lengend Snippet: Effects of genome editing on HIF target gene expression. a VEGFA mRNA expression in CRISPR/Cas9 genome edited Hep3B cells cultured in Lumox ® gas permeable petri-dishes (corresponding to Fig. ). Values are presented as mean ± SEM of n = 3 independent experiments. b Reporter gene assay. Ratios of Firefly/Renilla luciferase activity were measured in normoxic/hypoxic Hep3B cells after 8 h and normalised to normoxic control cells (Ctrl. (N)). Values are presented as mean ± SEM of n = 3 independent experiments. N normoxia, H hypoxia

Article Snippet: Human hepatocellular carcinoma Hep3B cells (ATCC) were maintained in RPMI 1640 medium (Gibco) containing 10% FBS and Penicillin/Streptomycin.

Techniques: Targeted Gene Expression, Expressing, CRISPR, Cell Culture, Reporter Gene Assay, Luciferase, Activity Assay, Control

Journal: Cancer Cell International

Article Title: ARNT is a potential direct HIF-1 target gene in human Hep3B hepatocellular carcinoma cells

doi: 10.1186/s12935-017-0446-2

Figure Lengend Snippet: ChIP assays of genome-edited Hep3B cells exposed to hypoxia (3% O 2 , 5 h). Values are presented as mean ± SEM of n = 3 independent experiments. IgG normal rabbit IgG, H3 histone H3

Article Snippet: Human hepatocellular carcinoma Hep3B cells (ATCC) were maintained in RPMI 1640 medium (Gibco) containing 10% FBS and Penicillin/Streptomycin.

Techniques:

Journal: Cancer Cell International

Article Title: ARNT is a potential direct HIF-1 target gene in human Hep3B hepatocellular carcinoma cells

doi: 10.1186/s12935-017-0446-2

Figure Lengend Snippet: Proposed mechanism of hypoxia-inducible ARNT expression in Hep3B cells

Article Snippet: Human hepatocellular carcinoma Hep3B cells (ATCC) were maintained in RPMI 1640 medium (Gibco) containing 10% FBS and Penicillin/Streptomycin.

Techniques: Expressing

Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Article Title: How Similar is Similar Enough? A Sufficient Similarity Case Study with Ginkgo biloba Extract

doi: 10.1016/j.fct.2018.05.013

Figure Lengend Snippet: Ginkgo biloba extract case study overview.

Article Snippet: , Attagene HepG2 reporter gene assays , 1, 1A, 1F, A-Z, constituents.

Techniques: In Vitro, In Vivo, Comparison